Composite
pqq
Part:BBa_K4383045
Designed by: Junxiao Zhang Group: iGEM22_SCU-China (2022-10-10)
J23100-B0034-pqqA-pqqB-pqqC-pqqD-pqqE-pqqF
pqq synthetic gene cluster under the control of J23100
figure1.pqq synthetic pathway
figure2.pqq production in E. coli DH5a
figure3.pqq production in E. coli BL21
figure4.pqq production in E. coli ECN
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 872
Illegal PstI site found at 1537
Illegal PstI site found at 1745
Illegal PstI site found at 1805
Illegal PstI site found at 1915
Illegal PstI site found at 2221
Illegal PstI site found at 3213 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 872
Illegal PstI site found at 1537
Illegal PstI site found at 1745
Illegal PstI site found at 1805
Illegal PstI site found at 1915
Illegal PstI site found at 2221
Illegal PstI site found at 3213 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2370
Illegal BamHI site found at 1353
Illegal XhoI site found at 3190 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 872
Illegal PstI site found at 1537
Illegal PstI site found at 1745
Illegal PstI site found at 1805
Illegal PstI site found at 1915
Illegal PstI site found at 2221
Illegal PstI site found at 3213 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 872
Illegal PstI site found at 1537
Illegal PstI site found at 1745
Illegal PstI site found at 1805
Illegal PstI site found at 1915
Illegal PstI site found at 2221
Illegal PstI site found at 3213
Illegal NgoMIV site found at 215
Illegal NgoMIV site found at 404
Illegal NgoMIV site found at 881
Illegal NgoMIV site found at 967
Illegal NgoMIV site found at 2015
Illegal NgoMIV site found at 2068
Illegal NgoMIV site found at 2539
Illegal AgeI site found at 1260 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4008
Illegal BsaI.rc site found at 1981
Illegal SapI.rc site found at 2866
Illegal SapI.rc site found at 4624
[edit]
Categories
Parameters
None |